Anti-perinuclear Factor as Diagnostic Marker in Rheumatoid Arthritis
Published: September 1, 2015 | DOI: https://doi.org/10.7860/JCDR/2015/.6456
Zeinab Abedian, Masoud Sagafi, Saeid abedian kenari, Farshideh Abedian
1. Researcher, Cellular and Molecular Biology Research Center, Babol University of Medical Sciences, Babol, Iran.
2. Professor, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
3. Associate Professor, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
4. Lecturer, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
Correspondence
Dr. Farshideh Abedian,
Lecturer, Department of Immunology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
E-mail: farshideh1340@gmail.com
Introduction: Rheumatoid Arthritis (RA) is a systemic autoimmune disease. It is associated with several auto antibodies which can serve as diagnostic and prognostic markers.
Aim: In this study, Anti perinuclear Factor (APF) was evaluated as a biomarker in comparison with Rheumatoid Factor (RF) in Rheumatoid Arthritis.
Materials and Methods: Fifty two sera of patients with RA (mean age 48±15.8), 23 sera of Patient control group (mean age 32.5 ± 16.9) and 30 sera of Healthy control group (mean age 32.1± 16.9) were analysed. The method is based on the binding of APF to perinuclear keratohyalin granules of buccal mucosal cell and its detection using a fluorescently labeled anti human total antiserum.
Results: APF were found in 71.2 % (37/52) of patients with RA. The sensitivity and specificity for APF from 1/5 serum dilution was 71.2% and 94.3% respectively. RF test had higher sensitivity (88.5%) compare to the APF test (71.2%), but its specificity was (86.8%) less than APF (94.3%).
There was no significant relationship between the onset of APF and severity of disease but there was significant relationship between the APF titer and severity of disease (p<0.05).
Conclusion: It is concluded that APF test is a valuable serological tool for the diagnosis of the disease and a useful serological marker to differentiate from the other inflammatory rheumatoid diseases.
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